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A DNA test for L2-HGA in Staffordshire Bull Terriers.



What is L2-HGA?

DNA test for L2-HGA

The researchers of the genetics team at the Animal HealthTrust (UK) have identified the specific mutation that causes the L2-HGA problem in Staffordshire Bull Terriers. The Trust's research group developed a DNA test for the disease. The disorder shows an autosomal recessive mode of inheritance: two copies of the defective gene (one inherited from each parent) have to be present for a dog to be affected by the disease. Individuals with one copy of the defective gene and one copy of the normal gene - called carriers - show no symptoms but can pass the defective gene onto their offspring. When two apparently healthy carriers are crossed, 25% (on average) of the offspring will be affected by the disease, 25% will be clear and the remaining 50% will themselves be carriers. Those carriers could not be detected by the tests previously available that involved either a blood or urine test detecting elevated levels of L2-hydroxyglutarate or magnetic resonance imaging.

It now is possible to identify at a young age and without doubt, all dogs that are capable of passing on the disease. The DNA test for the L2-HGA yields three possible outcomes:
  • Your dog is "clear", it has two healthy alleles, it will not develop L2-HGA and, just as important, it won't pass on the defect to the next generation.
  • Your dog is a carrier, it has one "healthy" allele and one defective one, it will not develop L2-HGA and, very important, it will pass on the defect to half of its offspring.
  • Your dog is affected and has two defective alleles, it will develop L2-HGA later in life and it will pass on the defective allele to its entire offspring in the next generation.


  • Applying this DNA test allows breeders to classify their potential breeding animals at a young age as "affected", "carrier" or "genetically healthy" in order to select effectively against the genetic disorder.

    Breeds at risk

    Breeding policy

    Testing for L2-HGA